Dr. CN Wang
Interests:Explore the frontier of molecular behavior
Father of two kids and with a family of two dogs, four rabbits, and an aquarium of fishes.
Hobbies: Reading, Travel, Gardening, and Singing
Interests: Explore the frontier of molecular behavior
Ph.D. in molecular biophysicist, pioneering differential DNA energetic theory and its application for oncology, perinatology, epidemiology, and neurodegenerative disorders. Inventor of Ligase Chain Reaction (LCR), FRET qPCR, Random-Access Cross-strand Hybridization (RACH) and Allelic Lateral Flow Assay (ALFA), etc.
• Ligase Chain Reaction (LCR)
• FRET Hemi-Nest-qPCR (AmpliSensor qPCR)
• Differential Energetic qPCR
• Fully Automated FRET-qPCR System
• Random Apurinic Crossover Hybridization (RACH)
• Allelic Lateral Flow Assay (ALFA)
• Body and Brain DNAge Clock Assay
• Mendelian Wide-ranging Kinship Assay
• Dizygotic Twins Fetal Sex Determination Assay
• Type-Specific Cancer Detection Test
• Invented and patented AmpliSensor PCR – a FRET-qPCR technique by hemi-nested amplification process.
• Invented Biphasic Liquid Interfacial Crossover Hybridization – a conformation sensitive nucleic acid quasi-amplification and detection processes based on interfacial self-propagating hybridization mechanism.
• Invented Random Apurinic Crossover Hybridization (RACH) – a novel label-free, capture-free nucleic acid biosensor system, which offers a simple and ultrasensitive platform for real-time detection of multi-analytes without target amplification and separation.
• Invented Allelic Lateral Flow Assay (ALFA) – A real-time finger DNA molecular assay by lateral flow process.
• Developed and marketed AmpliSensor Minilyzer – a 96 well thermoplate bottom-reading fluorometer.
• Developed and marketed AmpliSensor Analyzer – an integrated 96 well thermoplate fluorometer and reagent multi-dispensers.
• Developed and marketed AG8800 RoboGenie– an integrated 96 well plate thermocycler and fluorometer. (Ref. Certif. No. DE3-3062M1)
• Developed and marketed AG9900 RoboMaster– an automated qPCR instrument with integrated 96 well thermoplate fluorometer, thermocycler and multiple reagent dispensers. (Ref. Certif. No. DE3-3062M1)
• Obtained medical device certificate for AG9900 RoboMaster by State Food and Drug Administration of China. (Reg. No. SFDA (1) 20043401106)
• Distributed AmpliSensor qPCR systems to more than 15 countries worldwide.
• AmpliSensor Assay Application Program and the cycle-dosage equation for AmpliSensor-qPCR.
• AmpliSensor all-in-one qPCR kits for the following etiological agents: HIV, HCV, HBV, EBV, CMV, HPV, TB, HTLV-I, P. falciparum, C. trachomatis, H. pylori, Parvovirus B19, PBFD, BVDV, COVID-19, FIP etc.
SELECTED PUBLICATION OF AMPLISENSOR QPCR
Wang CNJ, Hogan M, Austin RH. (1982) DNA motions in the nucleosome core particles. Proc Natl Acad Sci USA. 79 (19):5896-900.
Hogan M, Wang CNJ, Austin RH. (1983) Triplet anisotropy decay measurements of DNA internal motion. Ciba Found Symp. 93:226-45.
Hogan ME, Hayes B, Wang NCJ, Austin RH. (1986) Ion induced DNA structure change in nucleosomes. Biochemistry. 25 (18): 5070-82.
Hogan M, Wang CNJ, Austin RH, Monitto CL, Hershkowitz. (1982) Molecular motion of DNA as measured by triplet anisotropy decay. Proc Natl Acad Sci USA.79 (11): 3518-22.
Wu, K.Y., Lee, C.H., Chong, S.K.F., Fritzgerald;,J.F., Wang, C.N. John. (1992) A DNA based, homogeneous assay for CMV detection. Abstract #117, Advanced Program. AAAS Science Innovation’92. New Techniques and instruments in biomedical research, San Francisco, July 21-25, 1992. p. 99.
Wu, K.Y., Wang, H.T. and Wang, C.N. John. (1994) Application of AmpliSensor Assay in Diagnosis of Infectious Diseases and Genetic Disease. Conference on New Horizons in Gene Amplification Technologies, San Francisco.
Wu, K.Y., Wang, H.T. and Wang, C.N. John. (1994) AmpliSensor System for High-Resolution PCR Quantitation. Conference on New Horizons in Gene Amplification Technologies, San Francisco.
Wang CNJ, Wu KY, Wang H-T (1995) Quantitative PCR Using the Amplisensor Assay. in PCR Primer: A Laboratory Manual, edited by Dieffenback, C.W. and Dveksler, G.S. p.193-202 Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York .
Chen, S, Yee A, Griffiths M, Wu KY, Wang CN, Rahn K, De Grandis SA. (1997) A rapid, sensitive and automated method for detection of Salmonella species in foods using AG-9600 AmpliSensor Analyzer. J Appl Microbiol. 83(3): 314-21.
Wang, C.N. High Resolution PCR (1997) Quantitation by AmpliSensor Assay Conference on Signal Detection and Amplification, San Diego.
Wang CNJ (1999) High Resolution PCR Quantitation by Amplisensor Assay in Quantitative PCR Protocols edited by Kochanowski, B., Reischl, U. p.245-251 Humana Press Inc. Totowa, NJ.
Ling, B., Schwartz CE, Friez M, Gao J, Page C, Rimsky L, Stevenson RE, Wang CN. (2003). Development of an Automated AmpliSensor QPCR System for Fragile X Newborn Screening. Greenwood Genetic center and Biotronics Corp. Abstract, XLMR11 Program, Pathos (Cyprus).
Yokoi H, Nonoguchi K, Kishishita M, Iwai M, Higashitsuji H, Fujita J. Use of AmpliSensor to quantitate gene expression in small amounts of samples: comparison with the quantitative RT-PCR method using CCD imaging system. Rinsho Byori. 1996 Sep; 44(9):847-52. Japanese.
Chiang PW, Song WJ, Wu KY, Korenberg JR, Fogel EJ, Van Keuren ML, Lashkari, Kurnit DM. Use of a fluorescent-PCR reaction to detect genomic sequence copy number and transcriptional abundance. Genome Res. 1996 Oct; 6(10): 1013-26.
Tan Y, Li Y, Tan S. Detection of mycobacterium tuberculosis by AmpliSensor-PCR technique and its clinical application. Zhonghua Jie He He Hu Xi Za Zhi. 1998 Feb;21(2):79-81. Chinese.
Cairns JP, Chiang PW, Ramamoorthy S, Kurnit DM, Sidransky D. A comparison between microsatellite and quantitative PCR analyses to detect frequent p16 copy number changes in primary bladder tumors. Clin Cancer Res. 1998 Feb; 4(2): 441-4.
Chen S, Xu R, Yee A, Wu KY, Wang CN, Read S, De Grandis SA. An automated fluorescent PCR method for detection of shinga toxin-producing Escherichia coli in foods. Appl Environ Microbiol. 1998 Nov; 64(11):4210-6.
Rautenberg P. Lubbert C, Weers W, Boetel E, Schweichler J, Zhou. Evaluation of the AmpliSensor PCR and the SHARP signal detection system for the early prediction of symptomatic CMV infection in solid transplant recipients. J Clin Virol. 1999 Jun;13 (1-2): 81-94.
Etoh K, Yamaguchi K, Tokudome S, Watanbe T, Okayama A, Stuver S, Mueller N, Takatsuki K, Matsuoka. Rapid quantification of HTLV-I provirus load; detection of monoclonal proliferation of HTLV-I-infected cells among blood donors. Int J Cancer. 1999 Jun 11;81(6):859-64.
Tan Y, Li Y, Zhang Y. Quantitative detection of Mycobacterium tuberculosis DNA in peripheral blood from patients with pulmonary tuberculosis by Amplisensor-PCR technique. Zhonghua Jie He He Hu Xi Za Zhi. 1999 Aug;22(8):481-3. Chinese.
Zhou X, Gao Z, Yao J. Relationship between HBV DNA serum level and acute exacerbation of the disease in chronic hepatitis B patients. Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi. 1999 Dec; 13(4): 335-9. Chinese.
Pei-Wen Chaing, Anna Schneider, Stephane Borgnat, Marie-Pierre Gaub, Pierre Oudet, David M. Kurnit, Didier Jacqmin. Molecular Analysis of Urine Sediment for Follow-up of Urinary Tract Cancers. Journal of the National Cancer Institute. 2000 Nov 1; Vol. 92, No.21:1779-1780.
Nie Q, Wang P, Zhou Y. Quantitation of hepatitis C virus RNA in amniotic fluid of gravida infected by hepatitis C virus. Zhonghua Fu Chan Ke Za Zhi. 2002 Jan; 37(1): 19-21. Chinese.
Matera G, Lamberti A, Quirino A, Foca D, Giancotti A, Barreca GS, Guadagnino V, Liberto MC. Changes in the prevalence of hepatitis C virus (HCV) genotype 4 in Calabria, Southern Italy. Diagn Microbiol Infect Dis. 2002 Mar;42(3):169-73.
Chen YX, Huang AL, Qi ZY, Guo SH. Establishment and assessment of two methods for quantitative detection of serum duck hepatitis B virus DNA. World J. Gastroenterol. 2004 Sept 15; 10(18): 2666-9.